Coding

Part:BBa_K1031911:Design

Designed by: He Shuaixin   Group: iGEM13_Peking   (2013-09-01)


XylS-Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 32
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 754
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 217


Design Notes

Molecular cloning to add terminator to XylS coding sequence.


Source

XylS comes from Pseudomonas putida, sequence found through Genbank: http://www.ncbi.nlm.nih.gov/nuccore/M10143.1

References